Glutathione S-transferase / GST Protein (Native)

Produkt-Information von Glutathione S-transferase / GST Protein

Glutathione S-transferase / GST Protein QC Testing

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: GST protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met 1
Molecular Mass	The recombinant Glutathione S-transferase (GST) consists of 229 amino acids and predicts a molecular mass of 26.9 kDa. The apparent molecular mass of GST is approximately 28 kDa in SDS-PAGE under reducing conditions
Formulation	Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 2mM GSH, pH 8.0 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von Glutathione S-transferase / GST Protein

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Beschreibung von Glutathione S-transferase / GST Protein

Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.

References

1. Douglas, KT . et al., 1987, Adv. Enzymol. Relat. Areas Mol. Biol. 59: 103 - 67. 
2. Beckett, GJ, et al., 1993,  Adv Clin Chem. 30: 281-380.
3. Wilce, MC. et al., 1994,  Biochim. Biophys. Acta. 1205 (1): 1-18.
4. Leaver, MJ. et al., 1998,  Marine Environmental Research. 46 (1-5): 71-74.

Sino Biological Inc. liefert Glutathione S-transferase / GST Protein (Native) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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