ErbB4 / HER4 Protein

29.05.2012

Produkt-Information von ErbB4 / HER4 Protein

Synonym	ERBB4, HER4
Protein Construction	A DNA sequence encoding the human ERBB4(NP_005226.1) (Met1-Arg649 ) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von ErbB4 / HER4 Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: ErbB4 / HER4 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gln 26
Molecular Mass	The recombinant human ERBB4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 865 amino acids and has a predicted molecular mass of 96.6 kDa. The apparent molecular mass of the protein is approximately 117 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of Sterile PBS,pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ErbB4 / HER4 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ErbB4 / HER4 Protein

ErbB4, also known as Her4, is a member of the tyrosine kinase receptors (RTK) and the epidermal growth factor receptor (EGFR) subfamily, and appears to play important roles in differentiation, development and certain carcinomas. It is a single transmembrane-span type I glycoprotein structurally consisting of multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 (PI-3) kinase binding site and a PDZ domain binding motif. However, the last two domains are absent in one of the two naturally occurring ErbB4 isoforms, ErbB4 CYT-2. ErbB4 ligands include the neuregulins, betacellulin and heparin-binding EGF-like growth factor (HB-EGF). Typically, heterodimerization of ErbB4 with ErbB2 forms the high affinity receptor complex, and subsequnetly transmit cellular signals by activating downstream src homology-2 (SH2) and phosphotyrosine binding (PTB) domain-containing signaling molecules.. ErbB4 undergoes sequential processing by TNF-α converting enzyme (TACE) and presenilin-dependent gamma-secretase to release the ErbB4 ICD (4ICD) with the NLS sequence which is essential for nuclear accumulation of 4ICD. It has been shown that the ligand-indued proteolytic process of ErbB4 results in the accumulation of 4ICD and cytochrome c efflux, and directly couples a cell surface signal to the tumor cell mitochondrial apoptotic pathway. Accordingly, in contrast to the other three members of the EGFR family, ErbB4 expression seems to be selectively lost in tumors with aggressive phenotypes.

References

Elenius K. et al., 1997, EMBO J. 16: 1268-78
Vidal GA. et al., 2005, J Biol Chem. 280: 19777-83.
Carpenter G. 2003, Exp Cell Res. 284: 66-77.
Vidal GA. et al., 2007, Oncogene. 26: 462-6.
Kainulainen V. et al., 2000, J Biol Chem. 275: 8641-9.
Naresh A. et al., 2006, Cancer Res. 66: 6412-20.

Sino Biological Inc. liefert ErbB4 / HER4 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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