ErbB3 / HER3 (aa 730-1065) Protein (GST Tag)

29.05.2012

Produkt-Information von ErbB3 / HER3 Protein

Synonym	HER3，LCCS2，ErbB-3，c-erbB3，erbB3-S，MDA-BF-1，MGC88033，c-erbB-3， p180-ErbB3，p45-sErbB3，p85-sErbB3
Protein Construction	A DNA sequence encoding the cytoplasmic domain (Pro 730-Ser 1065) of human ErbB3 (NP_001973.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von ErbB3 / HER3 Protein

Purity	> 82 % as determined by SDS-PAGE	SDS-PAGE: ErbB3 / HER3 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human ErbB3 /GST chimera consists of 573 amino acids and has a calculated molecular mass of 65 KDa. It migrates as an approximately 65 KDa band as predicted in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 50mM Tris,100mM NaCl, pH 7.5, 10%gly, 1mM GSH. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ErbB3 / HER3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ErbB3 / HER3 Protein

ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

References

Kraus MH. et al., 1989, Proc Natl Acad Sci. 86: 9193-7.
Carraway K.L. et al., 1994, J Biol Chem. 269: 14303 -6.
Wallasch C. et al., 1995, EMBO. J. 14: 4267-75.
Alimandi M. et al., 1995, Oncogene. 10:1813-21.
Gamett DC. et al., 1995, J Biol Chem. 270: 19022-7.
Katoh M. et al., 1993, Biochem Biophys Res Commun.192: 1189-97.

Sino Biological Inc. liefert ErbB3 / HER3 (aa 730-1065) Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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