ErbB2 / HER2 / CD340 (676-1255) Protein (GST Tag)

26.03.2012

Produkt-Information von ErbB2 / HER2 / CD340 Protein

Synonym	rbB2, NEU, NGL, HER2, TKR1, CD340, HER-2, c-erb B2, HER-2/neu
Protein Construction	A DNA sequence encoding the cytoplasmic domain (Lys 676-Val 1255) of human ErbB2 (NP_004439.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von ErbB2 / HER2 / CD340 Protein

Purity	> 82 % as determined by SDS-PAGE	SDS-PAGE: ErbB2 / HER2 / CD340 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gln 26
Molecular Mass	The recombinant human ErbB2 (676-1255)/GST chimera consists of 817 amino acids and predictes a molecular mass of 92 kDa. It migrates as an approximately 100 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0, 2mM GSH,10% gly Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

ErbB2 / HER2 / CD340 Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Verwandte Produkte & Themen von ErbB2 / HER2 / CD340 Protein

Related Areas:

Enzyme>>Protein Kinase>>Receptor Tyrosine Kinase>>HER2/ErbB2

Cancer>>Cancer Biomarkers>>HER2/ErbB2

Cancer>>Growth Factor & Receptor>>EGF Family & Receptor>>HER2/ErbB2

Cancer>>Growth Factor & Receptor>>Receptor Tyrosine Kinase>>HER2/ErbB2

Immunology>>Cluster of Differentiation>>Other>>HER2/ErbB2/CD340

Proteins:

HER2/ErbB2	Human	HER2/ErbB2/Fc Protein, Recombinant	10004-H02H
HER2/ErbB2	Human	HER2/ErbB2 Protein, Recombinant	10004-H08H
ErbB2/HER2	Mouse	ERBB2 / HER2 Protein, Recombinant	50714-M02H
ErbB2/HER2	Mouse	ErbB2/HER2 Protein, Recombinant	50714-M08H
HER2/ErbB2	Rat	HER2/Fc Protein, Recombinant	80079-R02H
HER2/ErbB2	Rat	HER2 Protein, Recombinant	80079-R08H
HER2/ErbB2	Rat	HER2 Protein, Recombinant	80079-RCCH
HER2/ErbB2	Rhesus	HER2/Fc Protein, Recombinant	90020-K02H
HER2/ErbB2	Rhesus	HER2 Protein, Recombinant	90020-K08H

Antibodies:

Human HER2/ErbB2	ELISA	Mouse Monoclonal Antibody	10004-MM01
Human HER2/ErbB2	ELISA	Mouse Monoclonal Antibody (Biotin conjugated)	10004-MM01B
Human HER2/ErbB2	WB	Mouse Monoclonal Antibody (Biotin conjugated)	10004-MM02B
Human HER2/ErbB2	FCM	ErbB2 / HER2 / CD340 Antibody ( FITC )	10004-MM03-F
Human HER2/ErbB2	WB, ELISA, Flow Cytometry	HER2/ErbB2 Antibody, Rabbit MAb	10004-R002
Human HER2/ErbB2	WB, ELISA, IHC, Flow Cytometry	HER2/ErbB2 Antibody, Rabbit MAb	10004-R511
Human HER2/ErbB2	Flow Cytometry	HER2/ErbB2 Antibody, Rabbit MAb	10004-R511F
Human HER2/ErbB2	WB, ELISA	Rabbit Polyclonal Antibody	10004-RP03
Human HER2/ErbB2	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10004-RP04
Rhesus HER2/ErbB2	WB, ELISA	HER2 / ErbB2 Antibody	90020-MM01
Rhesus HER2/ErbB2	WB, ELISA	HER2 / ErbB2 Antibody (Antigen Affinity Purified)	90020-RP02

Beschreibung von ErbB2 / HER2 / CD340 Protein

Receptor tyrosine-protein kinase erbB-2, also known as epidermal growth factor receptor-related protein, proto-oncogene Neu, proto-oncogene c-ErbB-2, CD340 and ErbB2, is a single-pass type I membrane protein, cytoplasm protein and nucleus protein which belongs to the protein kinase superfamily, Tyr protein kinase family and EGF receptor subfamily. The ErbB protein family, also known as epidermal growth factor receptor (EGFR) family, is a family of four structurally related receptor tyrosine kinases (ErbB1/EGFR, ErbB2/HER2, ErbB3/HER3, ErbB4/HER4). The gene symbol, ErbB, is derived from the name of a viral oncogene to which these receptors are homologous: Erythroblastic Leukemia Viral Oncogene. Excessive ErbB signaling is associated with the development of a wide variety of types of solid tumor. Insufficient ErbB signaling in humans is associated with the development of neurodegenerative diseases, such as multiple sclerosis and Alzheimer's Disease. Mouse ErbB2/HER2 is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for rat ErbB2/HER2. It binds to the 5'-TCAAATTC-3' sequence in the MT-CO2 promoter and activates its transcription. ErbB2/HER2 is widely expressed in epithelial cells, and amplification and/or overexpression of ErbB2/HER2 has been reported associated with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate and ovarian cancers. ErbB2/HER2 cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. This receptor forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. ErbB2/HER2 mediates signalling pathways which involve mitogen-activated protein kinase and phosphatidylinositol-3 kinase, this receptor plays an key role in development, cell proliferation and differentiation.

References

Yamamoto, T. et al., 1986, Nature. 319: 230-234.
Nathan J. 2001, J. Biol. Chem. 276:42153-42161.
Codony-Seeervat, J. et al., 1999, Cancer Res. 59: 1196-1201.
Tzahar, E. et al., 1998, Biochim. Biophys. Acta. 1377: 25-37.
Kermit, L. et al, 1999, J. Biol. Chem. 274: 5263-5266.
Ying-Xin Fan. et al., 2008, J. Biol. Chem. 283: 1588-1596.

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