ENPP5 Protein (His Tag)

05.09.2012

Produkt-Information von ENPP5 Protein

Synonym	ENPP5
Protein Construction	A DNA sequence encoding the human ENPP5(Q9UJA9) (Met1-Gly429 )with a C-terminal polyhistidine tag was expressed.
Source	Human
Expression Host	Human Cells

QC Testing von ENPP5 Protein

Purity	> 96 % as determined by SDS-PAGE	SDS-PAGE: ENPP5 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Pro 25
Molecular Mass	The recombinant human ENPP5 comprises 416 amino acids and has a predicted molecular mass of 48.1 kDa. The apparent molecular mass of the protein is approximately 81 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ENPP5 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ENPP5 Protein

ENPP5 belongs to the nucleotide pyrophosphatase/phosphodiesterase family(NPP) which catalyze the hydrolysis of phosphate diester bonds. NPP belongs to the alkaline phosphatase superfamily, which is a group of evolutionarily related enzymes that catalyze phosphoryl and sulfuryl transfer reactions. It is a family comprised by dimeric enzymes. There are seven isoforms in NPP family, some of which prefer nucleotide substrates, some of which prefer phospholipid substrates, and others of which prefer substrates that have not yet been determined. ENPP5 may play a role in neuronal cell communication. Hhowever, it lacks nucleotide pyrophosphatase and lysopholipase D activity. It may also be involved in neuronal cell communication. The amino acid sequence of human ENPP5 is 100%, 88%, and 82% identical to that of chimpanzee, dog and mouse/rat. It also belongs to a group of nucleotidemetabolizing ectoenzymes, which regulate the availability of extracellular nucleotides.

References

Strausberg RL. et al., 2002, Proc Natl Acad Sci. 99 (26): 16899-903.
Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
Lamesch P. et al., 2007, Genomics. 89 (3): 307-15.

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