Cystatin B / CSTB Antikörper (Antigen Affinität reinigt)

13.03.2012

Produkt-Information von Cystatin B / CSTB Antikörper

Immunogen	Recombinant Human Cystatin B / CSTB protein (Catalog#10435-H07E)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Human Cystatin B / CSTB (rh Cystatin B / CSTB; Catalog#10435-H07E; P04080; Met 2-Phe 98). Cystatin B / CSTB specific IgG was purified by Human Cystatin B / CSTB affinity chromatography.

Cystatin B / CSTB Antikörper Usage Guide

Specificity	Human Cystatin B / CSTB
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CSTB in WB. Using a DAB detection system, the detection limit for Human CSTB is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CSTB. The detection limit for Human CSTB is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von Cystatin B / CSTB Antikörper

Cystatin-B, also known as CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB and CST6, is a cytoplasm and nucleus protein which belongs to thecystatin family. Cystatin-B / CSTB is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Cystatin-B / CSTB is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Cystatin-B / CSTB is also thought to play a role in protecting against the proteases leaking from lysosomes. Defects in Cystatin-B / CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) which is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).

References

Pernu,H. et al., 1990, Acta Histochem. 88 (1): 53-7.
Martin J.R., et al., 1995, J. Mol. Biol. 246: 331-343.
Brannvall,K. et al., 2003,Biochem Biophys Res Commun. 308 (2): 369-74.
Rivenbark,A.G. et al., 2006, Lab Invest. 86 (12): 1233-42.

Sino Biological Inc. liefert Cystatin B / CSTB Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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