CD4 Antikörper (Antigen Affinity Purified)

13.03.2012

Produkt-Information von CD4 Antikörper

Immunogen	Recombinant Ferret CD4 protein (Catalog#60003-F08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Ferret CD4 (rF CD4;Catalog#60003-F08H; ABS50090.1; Met 1-Leu 401). CD4 specific IgG was purified by Ferret CD4 affinity chromatography.

CD4 Antikörper Usage Guide

Specificity	Ferret CD4
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Ferret CD4 in WB. Using a DAB detection system, the detection limit for Ferret CD4 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Ferret CD4. The detection limit for Ferret CD4 is approximately 0.00975 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von CD4 Antikörper

T-cell surface glycoprotein CD4, also known as T-cell surface antigen T4/Leu-3, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoproteinexpressed on the surface of T helper cells, regulatory T cells,monocytes, macrophages, anddendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 before being named CD4 in 1984. CD4 is aco-receptorthat assists theT cell receptor (TCR) to activate its T cell following an interaction with anantigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly withMHC class II molecules on the surface of the antigen presenting cell via itsextracellulardomain. CD4 is a primary receptor used byHIV-1to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

References

Ryu SE, et al., 1994, Structure2 (1): 59-74.
Isobe M, et al., 1986, Proc. Natl. Acad. Sci.83 (12): 4399-402.
Ansari-Lari MA, et al., 1996, Genome Res.6 (4): 314-26.
Hawash, et al., 2002, Biochim. Biophys. Acta. 1589 (2): 140-50.

Sino Biological Inc. liefert CD4 Antikörper (Antigen Affinity Purified) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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