Cynomolgus TNFRSF14 / HVEA / HVEM Protein (Cytokine)

29.03.2013

Sino Biological Inc. liefert Cynomolgus TNFRSF14 / HVEA / HVEM Protein (Cytokine) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus TNFRSF14 / HVEA / HVEM Protein (Cytokine) lesen Sie bitte: http://www.sinobiological.com/HVEM-TNFRSF14-Protein-a-463.html

Produkt-Information von TNFRSF14 / HVEA / HVEM Protein

Synonym	TNFRSF14
Protein Construction	A DNA sequence encoding the cynomolgus TNFRSF14 (Met1-Arg201) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

TNFRSF14 / HVEA / HVEM Protein QC Testing

Purity	(93.7+5.3)% as determined by SDS-PAGE	SDS-PAGE: TNFRSF14 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Leu 39
Molecular Mass	The recombinant cynomolgus TNFRSF14 is a disulfide-linked homodimer. The reduced monomer comprises 404 amino acids and has a calculated molecular mass of 44.2 KDa. The apparent molecular mass of the protein is approximately 57 and 36 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von TNFRSF14 / HVEA / HVEM Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von TNFRSF14 / HVEA / HVEM Protein

Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. Binding of herpes simplex virus (HSV) viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of HSV entry mechanism, and from which its name was derived. The short cytoplasmic region of HVEM interacts with members of the TNFR-associated factor (TRAF) family and mediates the signal transduction pathways that activate the immune response. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT ((TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA).

References

Montqomery RI. et al., 1996, Cell. 87: 427-36.
Marsters SA. et al., 1997, J Biol Chem. 272: 14029-32.
Schneider K. et al., 2004, Immunol Rev. 202: 49-66.
Cheung TC. et al., 2005, Proc Natl Acad Sci. 102: 13218-23.

Cytokine