Cynomolgus NT5E / CD73 Protein (His Tag)

21.03.2013

Sino Biological Inc. liefert Cynomolgus NT5E / CD73 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus NT5E / CD73 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CD73-NT5E-Protein-Antibody-a-941.html

Produkt-Information von NT5E / CD73 Protein

Synonym	NT5E
Protein Construction	A DNA sequence encoding the cynomolgus NT5E (Met1-Lys547) was expressed with a polyhistidine tag at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von NT5E / CD73 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: NT5E protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Trp 27
Molecular Mass	The recombinant cynomolgus NT5E comprises 532 amino acids and has a calculated molecular mass of 59.2 KDa. The apparent molecular mass of it is approximately 59 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von NT5E / CD73 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NT5E / CD73 Protein

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

References

Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.