Cynomolgus CD200 Protein

21.03.2013

Sino Biological Inc. liefert Cynomolgus CD200 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus CD200 Protein lesen Sie bitte: http://www.sinobiological.com/CD200-Protein-Antibody-a-332.html

Produkt-Information von CD200 Protein

Synonym	CD200
Protein Construction	A DNA sequence encoding the cynomolgus CD200 (NP_001244473.1) (Met1-Gly232) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von CD200 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CD200 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gln 31
Molecular Mass	The recombinant cynomolgus CD200 is a disulfide-linked homodimer. The reduced monomer comprises 443 amino acids and has a calculated molecular mass of 49.6 KDa. The apparent molecular mass of the protein is approximately 53-57 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CD200 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD200 Protein

CD200, also known as OX-2, is a transmembrane immunoregulatory protein belonging to the immunoglobulin superfamily. The mouse CD200 cDNA encodes a 278 aa precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain, and shares 76% and 94% aa sequence identity with human and rat CD200. CD200, a type 2 transmembrane molecule, plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion by delivering an immunoregulatory signal through interaction with its receptor. CD200 was found to be co-expressed with stem cell markers found on prostate, breast, brain, and colon cancers. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. CD200 and CD200R associate via their respective N-terminal Ig-like domains. In myeloid cells, CD200R initiates inhibitory signals following receptor-ligand contact. In T cells, CD200 functions as a costimulatory molecule independent of the CD28 pathway. CD200 and its interaction with the CD200 receptor is a potential therapeutic target for metastatic melanoma (MM).

References

Chen Z. et al., 1997, Biochim Biophys Acta. 1362 (1): 6-10.
Gorczynski RM. et al., 2001, Clin Exp Immunol. 126 (2): 220-9.
Shiratori I. et al., 2005, J Immunol. 175 (7): 4441-9.
Kawasaki BT. et al., 2007, Biochem Biophys Res Commun. 364 (4): 778-82.
Kretz-Rommel A. et al., 2008, J Immunol. 180 (2): 699-705.
Chen Z. et al., 2009, Mol Immunol. 46 (10): 1951-63.