Cynomolgus CTLA4 / CD152 Protein

20.03.2013

Sino Biological Inc. liefert Cynomolgus CTLA4 / CD152 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus CTLA4 / CD152 Protein lesen Sie bitte: http://www.sinobiological.com/CTLA-4-CD152-Protein-Antibody-a-500.html

Produkt-Information von CTLA4 / CD152 Protein

Synonym	CTLA4
Protein Construction	A DNA sequence encoding the cynomolgus CTLA4 (Q9BDC4) (Met1-Asp161) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von CTLA4 / CD152 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CTLA4 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 37
Molecular Mass	The recombinant cynomolgus CTLA4 is a disulfide-linked homodimer. The reduced monomer comprises 366 amino acids and has a calculated molecular mass of 40.4 KDa.The apparent molecular mass of the protein is approximately 52 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CTLA4 / CD152 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CTLA4 / CD152 Protein

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is widely expressed with highest levels in lymphoid tissues. CTLA4 contains a extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants encoding different isoforms have been characterized. CTLA4 is similar to the T-cell costimulatory protein CD28 since both of the molecules bind to CD80 and CD86 on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA4. Genetic variations of CTLA4 have been associated with susceptibility to systemic lupus erythematosus (SLE), Graves disease (GRD), Celiac disease type 3 (CELIAC3) and Hepatitis B virus infection ( HBV infection ).

References

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Magistrelli G. et al.,1999, Eur J Immunol. 29 (11): 3596-602.
Schneider H. et al., 2006, Science. 313 (5795): 1972-5.
Rudd CE. et al., 2009, Immunol Rev. 229 (1): 12-26.