Cynomolgus aFGF / FGF1 Protein (Native)

27.04.2013

Sino Biological Inc. liefert Cynomolgus aFGF / FGF1 Protein (Native) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus aFGF / FGF1 Protein (Native) lesen Sie bitte: http://www.sinobiological.com/aFGF-Protein-Antibody-a-195.html, /FGF-1-Protein-Antibody-a-192.html

Produkt-Information von aFGF / FGF1 Protein

Synonym	FGF1
Protein Construction	A DNA sequence encoding the cyno FGF1 (Phe16-Asp155) was expressed and purified with an initial Met.
Source	Cynomolgus
Expression Host	E.coli

QC Testing von aFGF / FGF1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: aFGF protein
Bio-activity	Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.1-0.4 ng/mL.
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant cyno FGF1 consists of 141 amino acids and has a calculated molecular mass of 16 kDa. It migrates as an 16 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4, 20% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von aFGF / FGF1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von aFGF / FGF1 Protein

aFGF (Acidic fibroblast growth factor), also known as FGF1 (Fibroblast growth factor 1), FGF-acidic and HBGF-1 (Heparin-binding growth factor 1), is a member of the fibroblast growth factor (FGF) family. It is a powerful mitogen exhibiting strong action on numerous different cell types. FGF1 protein plays a role in various stages of development and morphogenesis, as well as in angiogenesis and wound healing processes. aFGF protein binds to cell surface receptors with high affinity with the prerequisite association of aFGF with heparan sulfate. The aFGF gene is a repressed target of p53. It inhibits both p53-dependent apoptosis and cell growth arrest via an intracrine pathway. Accordingly, aFGF and p53 pathways may interact in the cell to determine cell fate.

References

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Gorczynski, R.M., 2005, Curr Opin Invest Drugs. 6: 483-7.
Frijns, C.J. et al., 2002, Stroke. 33 (8): 2115-22.
Bella, J. et al., 1998, Proc Natl Acad Sci. 95 (8): 4140-5.