CTSZ Protein (His Tag)

27.03.2012

Produkt-Information von CTSZ Protein

Synonym	CTSX
Protein Construction	A DNA sequence encoding the human CTSZ (Q9UBR2) (Met 1- Val 303) was fused with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von CTSZ Protein

Purity	> 97 % as determined by SDS-PAGE	SDS-PAGE: CTSZ protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly 24
Molecular Mass	The secreted recombinant human CTSZ (pro form) consists of 291 amino acids and has a predicted molecular mass of 33 kDa. The apparent molecular mass of rhCTSZ is approximately 40 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH7.4 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

CTSZ Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CTSZ Protein

CTSZ(cathepsin Z, Histidine-tagged) is a lysosomal cysteine proteinase and member of the peptidase C1 family. The family contains both endo- and exopeptidases. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The catalytic residues of family C1 have been identified as Cys and His, forming the catalytic dyad. Two other active site residues are found, a Gln residue preceding the catalytic Cys and an Asn residue following the catalytic His. The Gln is believed to help in the formation of the 'oxyanion hole' and the Asn to orientate the imidazolium ring of the catalytic His. There are a number of non-peptidase homologues in which catalytic residues have been replaced. CTSZ has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. CTSZ is widely expressed. Its release of C-terminal amino acid residues with broad specificity, but lacks action on C-terminal proline and it shows weak endopeptidase activity.

References

Santamaria I. et al., 1998, J Biol Chem. 273 (27): 16816-23.
Ngler DK. et al., 1998, FEBS Lett. 434 (1-2): 135-9.
Pungercar J. et al., 2000, Pflugers Arch. 439 (3 Suppl): R116-8.

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