CES3 / Carboxylesterase 3 Antikörper

24.02.2012

Produkt-Information von CES3 / Carboxylesterase 3 Antikörper

Immunogen	Recombinant Human CES3 / Carboxylesterase 3 protein (Catalog#11129-H08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Human CES3 / Carboxylesterase 3 (rh CES3 / Carboxylesterase 3; Catalog#11129-H08H; NP_079198.2; Met 1-Glu 561). Total IgG was purified by Protein A affinity chromatography.

CES3 / Carboxylesterase 3 Antikörper Usage Guide

Specificity	Human CES3 / Carboxylesterase 3
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CES3 in WB.
Direct ELISA	This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human CES3. The detection limit for Human CES3 is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von CES3 / Carboxylesterase 3 Antikörper

Carboxylesterases hydrolyze esters of short-chain fatty acids and have roles in animals ranging from signal transduction to xenobiotic detoxification. In enzymology, a carboxylesterase is an enzyme that catalyzes the chemical reaction: a carboxylic ester + pO = an alcohol + a carboxylate. Most enzymes from this group belong to the superfamily of hydrolases with alpha/beta protein fold (so called Alpha/beta hydrolase fold), specifically those acting on carboxylic ester bonds. The carboxylesterase family of evolutionarily related proteins (those with clear sequence homology to each other) includes a number of proteins with different substrate specificities, such as acetylcholinesterases. Carboxylesterase 3, also known as Liver carboxylesterase 31 homolog and CES3, is a endoplasmic reticulum lumen which belongs to thetype-B carboxylesterase/lipase family. CES3 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES3 shows low catalytic efficiency for hydrolysis of CPT-11, a prodrug for camptothecin used in cancer therapeutics. CES3 is expressed in liver, colon and small intestine.

References

Augusteyn RC, et al.,1969, Biochim. Biophys. Acta.171 (1): 128-37.
Saito S. et al., 2003, J. Hum. Genet. 48: 249-70.
Sanghani SP. et al., 2003, Clin. Cancer Res. 9: 4983-4991.
Sanghani SP. et al., 2004, Drug Metab. Dispos. 32: 505-511.
Chen R. et al., 2009, J. Proteome Res. 8: 651-61.

Sino Biological Inc. liefert CES3 / Carboxylesterase 3 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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