CD83 ELISA Pair Set, Human ELISA Pair Set

Fängerantikörper 0.5 mg/mL of mouse anti-CD83 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL mouse anti-CD83 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 7 ng of recombinant CD83. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 200 pg/mL is recommended.

The minimum detectable dose of Human CD83 was determined to be approximately 3.13 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Human CD83 ELISA Pair Set is for the quantitative determination of Human CD83.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD83 coated on a 96-well plate. Standards and samples are added to the wells, and any CD83 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-CD83 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD83 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

STORAGE - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

CD83 antigen, also known as B-cell activation protein, Cell surface protein HB15 and CD83, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD83 is a a conserved member of the immunoglobulin superfamily. CD83 is expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. CD83 is a fascinating cell surface molecule with a unique pattern of expression that has multiple confirmed functions in regulating immune system development and function. CD83 represents an intriguing target for immunotherapy due to its preferential expression on mature dendritic cells (DCs), the most efficient of antigen presenting cells. On the protein level, two different isoforms of CD83 exist: a membrane-bound and a soluble form. The isoforms are generated by different subsets of cells, including DCs, T-cells and B-cells, and also differ in their biological function. While the membrane-bound CD83 is of immune stimulatory capacity, activates T-cells and is important for the generation of thymocytes, the soluble CD83 has the opposite effect and has an immune inhibitory capacity. Due to its immune inhibitory function. CD83 may play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. CD83 has great potential for treatment of autoimmune diseases, for organ transplantations, and for immunotherapy, just to name a few examples. Moreover, some viruses prevent recognition by the host's immune system by specifically targeting CD83 surface expression.