CD40 / TNFRSF5 Antikörper ( FITC )

Produkt-Information von CD40 / TNFRSF5 Antikörper

Usage Guide von CD40 / TNFRSF5 Antikörper

Specificity	Human CD40 / TNFRSF5
	No cross-reactivity in ELISA with Human CD27 Mouse CD27 Human CD30 Human DR6 Human 4-1BB Human TNFRⅡ Human HVEM Mouse HVEM Human RELT Human cell lysate (293 cell line)
Flow Cytometry	Cells should be Fc-blocked by treatment with 10 μg of human IgG/106 cells for 1 hour at 4℃ prior to staining, washed, then stained with FITC Mouse anti-CD40 (MM03). Profile of CD40 reactivity on Daudi cells analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD40 in WB.
Storage	This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Verwandte Produkte & Themen von CD40 / TNFRSF5 Antikörper

Immunology>>Adaptive Immunity>>Costimulation & Costimulatory Molecule>>CD40/TNFRSF5

Immunology>>Cytokine & Receptor>>TNF Superfamily>>CD40/TNFRSF5

Immunology>>Cluster of Differentiation>>B Cell CD Antigen>> CD40/TNFRSF5

Immunology>>Cluster of Differentiation>>Monocyte/Macrophage CD Antigen>>Macrophage Markers / Monocyte Markers>>CD40/TNFRSF5

Immunology>>Cluster of Differentiation>>Natural Killer (NK) Cell CD Antigen>> CD40/TNFRSF5

Hintergrund von CD40 / TNFRSF5 Antikörper

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The cognate interaction between CD40 and CD40 ligand (CD154) on T cells activates NF-kappa B, Jun N-terminal kinase, and Janus kinase signal transducers and activators of transcription pathways. Several different TRAF proteins (Adaptor proteins) have been identified to serves as mediators of the signal transduction. In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.

References

1. Banchereau, J. et al., 1994, Annu. Rev. Immunol. 12: 881-922.
2. Ni, C.Z. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97: 10395-10399.
3. Ferrari.S. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 12614-12619.
4. Bhushan, A and Covey, L.R. 2001, Immunol. Res. 24: 311-324.

Sino Biological Inc. liefert CD40 / TNFRSF5 Antikörper ( FITC ) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CD40 / TNFRSF5 Antikörper ( FITC ) lesen Sie bitte: http://www.sinobiological.com/CD40-TNFRSF5-Antibody-g-8811.html