CD3E Protein

25.12.2012

Sino Biological Inc. liefert CD3E Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CD3E Protein lesen Sie bitte: http://www.sinobiological.com/CD3E-Protein-g-10181.html

Produkt-Information von CD3E Protein

Synonym	CD3E
Protein Construction	A DNA sequence encoding the human CD3E ( Met 1-Asp126) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von CD3E Protein

Purity	> (84.6+ 14.1) % as determined by SDS-PAGE	SDS-PAGE: CD3E protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gln 22
Molecular Mass	The secreted recombinant human CD3E is a disulfide-linked homodimeric protein. The reduced monomer comprises 355 amino acids and has a predicted molecular mass of 40 kDa. As a result of glycosylation, it migrates as an approximately 44-53 and 38 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CD3E Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Verwandte Produkte & Themen von CD3E Protein

Related Areas:

Cancer>>Cancer Stem Cell (CSC)>>CD3e

Immunology>>Adaptive Immunity>>T Cell>>Regulatory T Cell / T Cell Antigen Recognition>>CD3e

Immunology>>Cluster of Differentiation>>T Cell CD Antigen>>Regulatory T Cells / T Cell Antigen Recognition>>CD3e

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
CD3e/CD3 epsilon	Human	CD3E Protein, Recombinant	10977-H03H
CD3e/CD3 epsilon	Human	CD3e Protein, Recombinant	10977-H08H
CD3e/CD3 epsilon	Cynomolgus	CD3E Protein, Recombinant	90047-C02H
CD3e/CD3 epsilon	Cynomolgus	CD3E Protein, Recombinant	90047-C08H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human CD3e/CD3 epsilon	WB, ELISA	CD3e/CD3 epsilon Antibody, Mouse MAb	10977-MM03

Beschreibung von CD3E Protein

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

References

Wong S. et al., 1991, Clin Exp Immunol. 83 (1): 69-73.
Call ME. et al., 2004, EMBO J. 23 (12): 2348-57.
Arnett KL. et al., 2004, Proc Natl Acad Sci. 101: 16268-73.
Kjer-Nielsen L. et al., 2004, Proc Natl Acad Sci. 101: 7675-80.
Fischer A. et al., 2005, Curr Opin Allergy Clin Immunol. 5 (6): 491-5.
Holst J. et al., 2008, Nature immunology. 9 (6): 658-66.