CD247 / CD3-ZETA Protein (GST Tag)

10.10.2012

Produkt-Information von CD247 / CD3-ZETA Protein

Synonym	CD247, CD3Z, T3Z, TCRZ
Protein Construction	A DNA sequence encoding the mature form of human CD247 (P20963-1) (Arg52-Arg164) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von CD247 / CD3-ZETA Protein

Purity	> 92 % as determined by SDS-PAGE	SDS-PAGE: CD247 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human CD247 /GST chimera consists of 347 amino acids and has a predicted molecular mass of 40.2 kDa. It migrates as an approximately 28-43 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CD247 / CD3-ZETA Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD247 / CD3-ZETA Protein

CD247, also known as CD3-ZETA, contains 3 ITAM domains and is a member of the CD3Z/FCER1G family. It is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for CD247 gene. CD247 may play a role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.

References

Radstake TR. et al., 2010, Nat Genet. 42 (5): 426-9.
Dieudé P. et al., 2011, Ann Rheum Dis. 70 (9): 1695-6.
Li R. et al., 2012, Lupus. 21 (1): 75-83.

Sino Biological Inc. liefert CD247 / CD3-ZETA Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CD247 / CD3-ZETA Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/CD247-CD3-ZETA-Protein-g-9369.html