CD208 / DC-LAMP / LAMP3 ELISA Pair Set, Human ELISA Pair Set

Fängerantikörper 1 mg/mL of rabbit anti-LAMP3 monoclonal antibody, Dilute to a working concentration of 2.0 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL rabbit anti-LAMP3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use.

Standard Each vial contains 50 ng of recombinant LAMP3. Reconstitute standard powder with detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.

The minimum detectable dose of Human CD208 / DC-LAMP / LAMP3 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Human CD208 / DC-LAMP / LAMP3 ELISA Pair Set is for the quantitative determination of Human CD208 / DC-LAMP / LAMP3.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD208 / DC-LAMP / LAMP3 coated on a 96-well plate. Standards and samples are added to the wells, and any CD208 / DC-LAMP / LAMP3 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD208 / DC-LAMP / LAMP3 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD208 / DC-LAMP / LAMP3 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family. DC-LAMP and CD68 share very similar predicted structure and are the only two members of this LAMP family with a single membrane-proximal (domain II) lamp-like domain, and a region considered as a mucin-like structure rich in serines and threonines in the N-terminal portion (domain I) . DC-LAMP is highly glycosylated: the observed mass of the mature glycoprotein on SDS-PAGE is around 70–90 kDa, whereas the predicted mass for the polypeptide core is 44 kDa. The presence of DC-LAMP in the MIIC in late mature DC indicates that this may perform an important function during the processing of exogenous antigens, and might also participate in the functional remodeling of the MIIC by facilitating the translocation of MHC class II molecules to the cell surface.Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.