CCN3 / NOV / IGFBP9 ELISA Pair Set, Human ELISA Pair Set

Fängerantikörper 0.5 mg/mL of rabbit anti-NOV monoclonal antibody, Dilute to a working concentration of 2.0 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL mouse anti-NOV monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.05 μg/mL in detection antibody dilution buffer before use.

Standard Each vial contains 100 ng of recombinant NOV. Reconstitute standard powder with detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.

The minimum detectable dose of Human CCN3 / NOV / IGFBP9 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Human CCN3 / NOV / IGFBP9 ELISA Pair Set is for the quantitative determination of Human CCN3 / NOV / IGFBP9.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CCN3 / NOV / IGFBP9 coated on a 96-well plate. Standards and samples are added to the wells, and any CCN3 / NOV / IGFBP9 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-CCN3 / NOV / IGFBP9 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CCN3 / NOV / IGFBP9 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is secreted protein which belongs to the CCN family. CCN3 expression was observed in a broad variety of tissues from the early stage of development. It contains one CTCK (C-terminal cystine knot-like) domain, one IGFBP N-terminal domain, one TSP type-1 domain, and one VWFC domain. CCN3, a founding member of the CCN family of growth regulators, was linked with hematology in 2003 when it was detected in human serum. CCN3 acts through the core stem cell signalling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of a number of cell lineages including hematopoietic, osteogenic and chondrogenic. Altered CCN3 expression is associated with numerous solid tumors including glioblastoma, melanoma, adrenocortical tumours, prostate cancer and bone malignancies including osteosarcoma. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis and a crucial component within the bone marrow microenvironment.