CD147 / EMMPRIN / Basigin Antikörper ( FITC )

Sino Biological Inc. liefert CD147 / EMMPRIN / Basigin Antikörper ( FITC ) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CD147 / EMMPRIN / Basigin Antikörper ( FITC ) lesen Sie bitte: http://www.sinobiological.com/CD147-EMMPRIN-Antibody-g-6056.html

Produkt-Information von CD147 / Basigin Antikörper

Usage Guide von CD147 / Basigin Antikörper

Specificity	Human CD147 / Basigin / BSG
Flow Cytometry	Fig 1, Fig 2 and Fig 3. Flow cytometric analysis of anti-CD147 reactivity Profile of anti-CD147 reactivity on HeLa cells, PC-3 cells, A549 cells analyzed by flow cytometry Fig 1. Analysis of anti-CD147 reactivity on HeLa cells Fig 2. Analysis of anti-CD147 reactivity on PC-3 cells Fig 3. Analysis of anti-CD147 reactivity on A549 cells Fig 4. Profile of peripheral blood lymphocytes analyzed by flow cytometry The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Fig 4. Analysis of peripheral blood lymphocytes Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Immunochemistry	ICC/IF: 1-10 μg/mL      Immunofluorescence staining of human CD147 in HeLa cells with rabbit monoclonal antibody (5 µg/mL). The image showing membrane staining of HeLa cells. Primary antibody (FITC conjugated) was incubated for 1 hour at 37℃.
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD147 in WB.Using a DAB detection system, the detection limit for Human CD147 is approximately 0.25 ng/lane under non-reducing conditions and 5 ng/lane under reducing conditions.
Storage	This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !   Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Verwandte Produkte & Themen von CD147 / Basigin Antikörper

Enzyme>>Protease & Regulator>>Metalloprotease & Regulator>>CD147/EMMPRIN

Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hemangioblast>>Endothelial Cell Marker>>CD147/EMMPRIN

Cancer>>Cancer Biomarkers>>CD147/EMMPRIN

Immunology>>Adaptive Immunity>>T Cell>>T Cell Migration/Adhesion>>CD147/EMMPRIN

Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>CD147/EMMPRIN

Immunology>>Cluster of Differentiation>>Dendritic Cell CD Antigen>>Dendritic Cell Adhesion>>CD147/EMMPRIN

Immunology>>Cluster of Differentiation>>Granulocyte CD Antigen>>Basophils / Eosinophils / Granulocyte Markers /Neutrophils>>CD147/EMMPRIN

Immunology>>Cluster of Differentiation>>Monocyte/Macrophage CD Antigen>>Monocyte Markers>>CD147/EMMPRIN

Immunology>>Cluster of Differentiation>>T Cell CD Antigen>>T Cell Migration/Adhesion>>CD147/EMMPRIN

Hintergrund von CD147 / Basigin Antikörper

CD147, also known as Basigin, BSG, and EMMPRIN, is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. CD147 is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation, and tumor progression. Enriched on the plasma memebrane of many human cancer (breast cancer, lymphoma, oral squamous cell carcinoma, glioma, melanoma, lung, bladder and kidney carcinomas ) cells, CD147 induces the production and release of matrix metalloproteinases in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction.

References

1. Kanekura, T. et al., 1991, Cell. Struct. Funct. 16 : 23-30.
2. Muramatsu ,T. et al., 2003, Histol Histopathol. 18 : 981-7.
3. Jin-Ming Yang. et al., 2006, J. Biol. Chem. 281: 9719-27.
4. Hakuma, N. et al., 2007, Oncology. 72 : 197-204.
5. Zheng HC. et al., 2006, Br. J. Cancer. 95 : 1371-8.
6. Yurchenko,V. et al., 2002, J. Biol. Chem. 277 : 22959-65.