CD105 / Endoglin Antikörper ( FITC )

05.01.2012

Produkt-Information von Endoglin / CD105 Antikörper

Immunogen	Recombinant human CD105 protein ( Catalog#10149-H08H )
Reagents	FITC-conjugated mouse monoclonal antibody
Clone ID	4A3G3D3
Ig Type	Mouse IgG2b
Concentration	5 μl/Test, 0.2 mg/ml
Formulation	Aqueous solution containing 0.5% BSA and 0.1% sodium azide
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human Endoglin / CD105 ( rh CD105 ; Catalog#10149-H08H ; Met 1 - Gly 586 ; NP_001108225.1 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with FITC under optimum conditions, the unreacted FITC was removed

Usage Guide von Endoglin / CD105 Antikörper

Specificity

Human Endoglin / CD105 / ENG

Flow Cytometry

1. Flow cytometric analysis of CD105 expression on human HUVEC cells
HUVEC cells ( Human umbilical vein endothelial cells ) were stained with FITC Mouse anti-Human CD105 antibody ( solid line fluorescence histogram ) or a negative control at a matching concentration; dashed line histogram) antibody. Flow cytometric fluorescence histograms were derived from gated events with the forward and side light -scatter characteristics of viable cells.

Fig 1. Analysis of CD105 expression on human HUVEC cells

2. Flow cytometric analysis of CD105 expression on human HeLa cells
HeLa cells were stained with FITC Mouse anti-Human CD105 antibody ( solid line fluorescence histogram ) or a negative control at a matching concentration; dashed line histogram ) antibody. Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells.

Fig 2. Analysis of CD105 expression on human HeLa cells

Flow cytometry was performed on a BD FACSCalibur flow cytometry system

Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

Storage

This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Verwandte Produkte & Themen von Endoglin / CD105 Antikörper

Related Areas:

Neuroscience>>Axon Guidance>>TGF-beta Family>>CD105/Endoglin

Stem Cell>>Mesenchymal Stem Cell (MSC)>>Mesenchymal Stem Cell Marker>>CD105/Endoglin

Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hematopoietic Stem Cell Marker>>CD105/Endoglin

Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hemangioblast>>Hemangioblast Marker>>CD105/Endoglin

Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hemangioblast>>Endothelial Cell Marker>>CD105/Endoglin

Cancer>>Cancer Stem Cell (CSC)>>CD105/Endoglin

Cancer>>Growth Factor & Receptor>>TGF-beta Superfamily>>TGF-beta Family>>CD105/Endoglin

Immunology>>Cytokine & Receptor>>TGF-beta Superfamily>>TGF-beta Superfamily Receptors>>CD105/Endoglin

Immunology>>Cluster of Differentiation>>Hematopoietic Stem Cell CD Marker>>CD105/Endoglin

Immunology>>Cluster of Differentiation>>Monocyte/Macrophage CD Antigen>>Macrophage Markers>>CD105/Endoglin

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
CD105/Endoglin	Human	CD105/Endoglin/Fc Protein, Recombinant	10149-H02H
CD105/Endoglin	Human	CD105/Endoglin Protein, Recombinant	10149-H08H
CD105/Endoglin	Mouse	CD105/Endoglin Protein, Recombinant	50407-M08H

Antibodies:

Molecule	Application	Description //For Detailed Info.------CLICK!	Cat No
Human CD105/Endoglin	WB	CD105/Endoglin Antibody, Mouse MAb	10149-MM04
Human CD105/Endoglin	WB	Endoglin / CD105 Antibody	10149-MM09
Human CD105/Endoglin	ELISA	CD105/Endoglin Antibody, Mouse MAb	10149-MM11
Human CD105/Endoglin	FCM	Endoglin / CD105 Antibody ( FITC )	10149-MM11-F
Human CD105/Endoglin	WB, ELISA	CD105/Endoglin Antibody, Rabbit PAb	10149-RP01
Human CD105/Endoglin	WB, ELISA	CD105/Endoglin Antibody, Rabbit PAb (Antigen Affinity Purified)	10149-RP02
Human CD105/Endoglin	WB, ELISA	Endoglin / CD105 / ENG Antibody	10149-R001
Human CD105/Endoglin	WB, FCM	Endoglin / CD105 Antibody ( FITC )	10149-R103-F
Mouse CD105/Endoglin	WB, ELISA	CD105/Endoglin Antibody, Rabbit PAb	50407-RP01
Mouse CD105/Endoglin	WB, ELISA	CD105/Endoglin Antibody, Rabbit PAb (Antigen Affinity Purified)	50407-RP02
Mouse CD105/Endoglin	WB, ELISA	Endoglin / CD105 / ENG Antibody	50407-R001

Hintergrund von Endoglin / CD105 Antikörper

Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Endoglin contains an RGD tripeptide, a key recognition structure in cellular adhesion, and suggests a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type II receptor (TβRII) and activates the downstream signal pathway. In addition, ALK-1 is also a receptor kinase in human umbilical vein endothelial cells for endoglin threonine phosphorylation, and mutations in either result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful marker of neovascularization, and associated with several solid tumor types.

References

Cheifetz, S. et al., 1992, J. Biol. Chem. 267: 19027-19030.
McAllister, KA. et al., 1994, Nat. Genet. 8: 345-351.
Barbara, NP. et al., 1999, J. Biol. Chem. 274: 584-594.
Koleva, RI. et al., 2006, J. Biol. Chem. 281: 25110-25123.
Dallas, NA. et al., 2008, Clin. Cancer Res. 14:1931-1937.

Sino Biological Inc. liefert CD105 / Endoglin Antikörper ( FITC ) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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