CBLC / Cbl-c Protein (GST Tag)

14.05.2013

Sino Biological Inc. liefert CBLC / Cbl-c Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CBLC / Cbl-c Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/Cbl-c-CBL-3-Protein-Antibody-a-5920.html

Produkt-Information von CBLC / Cbl-c Protein

Synonym	CBLC, CBL3, RNF57
Protein Construction	A DNA sequence encoding the human CBLC (AAH28915.1) (Met1-Ala474) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von CBLC / Cbl-c Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CBLC protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human CBLC chimera consists of 711 amino acids and has a calculated molecular mass of 80.2 kDa. The recombinant protein migrates approximately 66 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CBLC / Cbl-c Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CBLC / Cbl-c Protein

CBL proteins, such as Cbl-c, are phosphorylated upon activation of a variety of receptors that signal via protein tyrosine kinases. Through interactions with proteins containing SRC homology-2 (SH2) and SH3 domains, CBL proteins modulate downstream cell signaling. Cbl-c is a member of the Cbl family of E3 ubiquitin ligases. Expression of Cbl-c gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for Cbl-c gene.

References

Kim M. et al., 1999, Gene. 239 (1): 145-54.
Courbard JR. et al., 2003, J. Biol. Chem. 277 (47): 45267-75.
Keane MM. et al., 1999, Oncogene. 18 (22): 3365-75.