CASQ1 / Calsequestrin Protein (Native)

18.04.2013

Sino Biological Inc. liefert CASQ1 / Calsequestrin Protein (Native) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CASQ1 / Calsequestrin Protein (Native) lesen Sie bitte: http://www.sinobiological.com/Calsequestrin-1-CASQ1-Protein-Antibody-a-5871.html

Produkt-Information von CASQ1 / Calsequestrin Protein

Synonym	CASQ1, CASQ
Protein Construction	A DNA sequence encoding the mature form of human CASQ1 (P31415) (Gln35-Asp396) was expressed with a N-terminal Met.
Source	Human
Expression Host	E.coli

QC Testing von CASQ1 / Calsequestrin Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: CASQ1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human CASQ1 consists of 363 amino acids and predicts a molecular mass of 41.8 KDa. It migrates as an approximately 52 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 10% glycerol, pH 7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CASQ1 / Calsequestrin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CASQ1 / Calsequestrin Protein

Calsequestrin-1 is an isoform of calsequestrin. Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. It helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. Two forms of calsequestrin have been identified: Calsequestrin-2 and Calsequestrin-1. Calsequestrin-1 is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50% of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin-1 is also secreted in the gut where it deprives bacteria of calcium ions.

References

Slupsky JR. et al., 1987, Biochemistry. 26 (20): 6539-44.
Cala SE. et al., 1991, J Biol Chem. 266 (1): 391-8.
Wang S. et al., 1998, Nat Struct Biol. 5 (6): 476-83.

Calsequestrin-1 (CASQ1) related areas, pathways, and other information