c-Kit / CD117 / KIT Antikörper (Antigen Affinität reinigt)

23.02.2012

Produkt-Information von c-Kit / CD117 / KIT Antikörper

Immunogen	Recombinant Human c-Kit / CD117 / KIT protein (Catalog#11996-H08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Human c-Kit / CD117 / KIT (rh c-Kit / CD117 / KIT; Catalog#11996-H08H; P10721-2; Met 1-Thr 516). c-Kit / CD117 / KIT specific IgG was purified by Human c-Kit / CD117 / KIT affinity chromatography.

c-Kit / CD117 / KIT Antikörper Usage Guide

Specificity	Human c-Kit / CD117 / KIT
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human KIT in WB. Using a DAB detection system, the detection limit for Human KIT is approximately 0.5 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human KIT. The detection limit for Human KIT is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von c-Kit / CD117 / KIT Antikörper

Mast / stem cell growth factor receptor, also known as Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117 and KIT, is a single-pass type I membrane protein which belongs to theprotein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. CD117 / KIT contains fiveIg-like C2-type (immunoglobulin-like) domains and oneprotein kinase domain. CD117 / KIT is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K). CD117 is the receptor for the cytokine stem cell factor (SCF), when this receptor binds to SCF it forms a dimer that activates signaling through second messengers. Signaling through CD117 plays a role in cell survival, proliferation, and differentiation. Defects in CD117 / KIT are a cause of piebaldism which is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. Defects in CD117 / KIT have also been associated with testicular tumors (TEST) which is a common solid malignancy in males.

References

Fleischman R.A. et al., 1992, J. Clin. Invest. 89:1713-1717.
Spritz R.A., et al., 1992, Am. J. Hum. Genet. 50:261-269.
Syrris P., et al., 2000, Am. J. Med. Genet. 95:79-81.
Leong KG, et al., 2008, Nature. 456. (7223): 804.

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