BTC / Betacellulin Protein

23.05.2013

Sino Biological Inc. liefert BTC / Betacellulin Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über BTC / Betacellulin Protein lesen Sie bitte: http://www.sinobiological.com/Betacellulin-Protein-a-934.html

Produkt-Information von BTC / Betacellulin Protein

Synonym	BTC
Protein Construction	A DNA sequence encoding the human BTC (P35070) (Met1-Tyr111) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Human
Expression Host	Human Cells

BTC / Betacellulin Protein QC Testing

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: BTC protein
Bio-activity	Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 5-40 ng/mL.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asp 32
Molecular Mass	The recombinant human BTC/Fc is a disulfide-linked homodimer. The reduced monomer comprises 321 amino acids and has a predicted molecular mass of 36 kDa. The apparent molecular mass of the protein is approximately 43-46 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von BTC / Betacellulin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von BTC / Betacellulin Protein

Probetacellulin, also known as Betacellulin and BTC is a single-pass type I membrane protein. Betacellulin is synthesized in several tissues and tumor cells. It is predominantly expressed in pancreas and small intestine. The expression of Betacellulin is particularly high in the pancreas where it is thought to play a role in the differentiation of pancreatic beta cells. Betacellulin belongs to the epidermal growth factor (EGF) family of peptide ligands that are characterised by a six-cysteine consensus motif that forms three intra-molecular disulfide bonds crucial for binding the ErbB receptor family. Betacellulin was initially described, purified and cloned from a mouse insulinoma cell line. It is proteolytically processed from a larger membrane-anchored precursor and is a potent mitogen for a wide variety of cell types. Betacellulin binds and activates ErbB-1 and ErbB-4 homodimers. Betacellulin stimulated gastrointestinal growth, the increased responsiveness of the terminal ileum and colon suggesting therapeutic potential in disease conditions in which ileal or colonic re-growth is desirable. Betacellulin further stimulated a diuresis suggesting an additional role in fluid homeostasis.

References

Dunbar AJ. et al., 2000, Int J Biochem Cell Biol. 32 (8): 805-15.
Howarth GS. et al., 2003, Growth Factors. 21 (2): 79-86.
Moon WS. et al., 2006, Hum Pathol. 37 (10): 1324-32.
Mummery RS. et al., 2007, Glycobiology. 17 (10): 1094-103.
Dunbar AJ. et al., 2001, J Mol Endocrinol. 27 (2): 239-47.