BID Antikörper ( FITC )

Produkt-Information von BID Antikörper

Usage Guide von BID Antikörper

Specificity	Human BID
	No cross-reactivity in ELISA with E.coli cell lysate
Flow Cytometry	The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Mouse anti-BID. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis of BID expression on Jurkat cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human BID in WB.
Storage	This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Verwandte Produkte & Themen von BID Antikörper

Cancer>>Apoptosis>>Bcl-2 Family>>BID

Hintergrund von BID Antikörper

BID, BH3-interacting domain death agonist, is a member of the Bcl-2 protein family. BID contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. It is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent, and also offer a new strategy for the treatment of acute renal failure associated with ischemia-reperfusion. BID receives direct inputs from a key regulator of the cell cycle arrest/DNA repair machinery (ATM), and therefore is an excellent candidate to coordinate genotoxic stress responses and apoptotic cell death. BID is a novel pro-apoptosis Bcl-2 family protein that is activated by caspase 8 in response to Fas/TNF-R1 death receptor signals. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. Recent studies further indicate that BID may be more than just a killer molecule. Deletion of BID inhibits carcinogenesis in the liver, although this genetic alteration promotes tumorigenesis in the myeloid cells. This is likely related to the function of BID to promote cell cycle progression into S phase. BID could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint.

References

1. Wei Q. et al., 2007, Ren Fail. 29 (8): 935-40.
2. Culmsee C. et al., 2006, Biochem Soc Trans. 34 (Pt 6): 1334-40.
3. Gross A. 2006, Cell Cycle. 5 (6): 582-4.
4. Yin XM. 2006, Gene. 369: 7-19.
5. Yin XM. 2000, Cell Res. 10 (3): 161-7.

Sino Biological Inc. liefert BID Antikörper ( FITC ) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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