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Beta-Tubulin Antikörper

Sino Biological Inc. liefert Beta-Tubulin Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Beta-Tubulin Antikörper lesen Sie bitte: http://www.sinobiological.com/Beta-Tubulin-Antibody-g-10214.html

Produkt-Information von Beta-Tubulin Antikörper

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Antibody Type Mouse Monoclonal Antibody ( Mouse mAb Service Platform )

Clone ID

05

Ig Type

 

Formulation 0.2 μm filtered solution in PBS with 5% trehalose
Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von Beta-Tubulin Antikörper

Specificity

Human Beta-Tubulin

Western blot

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human Beta-Tubulin in WB.

Beta-Tubulin Western blot
  A B
Sample
(whole cell lysate)
Hela MCF7
Sample Volume 20 μg/lane 5×105 cells/lane
Gel 13% SDS-PAGE reducing gel
Recommended Concentration 2-5 μg/ml
Secondary Antibody Dylight 800-labeled Antibody To Mouse IgG (H+L), at 1:20000 dilution.
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von Beta-Tubulin Antikörper

Related Areas:
Proteins:
Antibodies:

Hintergrund von Beta-Tubulin Antikörper

Beta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules. The dimers of alpha- and beta-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the beta -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Beta-tubulin faces the plus end of the microtubule while alpha-tubulin faces the minus end. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart.

References

  1. Williams RC Jr. et al., 1999. Anal Biochem. 275(2): 265-7.
  2. Nogales E. et al., 1998. Nat Struct Biol. 5(6): 451-8.
  3. Dutcher SK. 2001. Curr Opin Cell Biol. 13(1): 49-54.

Primary Antibody Products

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