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| Immunogen | Recombinant Human Bcl-XL / BCL2L1 protein (Catalog#10455-H08E) |
| Antibody Type | Mouse Monoclonal Antibody ( Mouse mAb Service Platform ) |
| Clone ID | 07 |
| Ig Type | Mouse IgG1 |
| Formulation | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Bcl-XL / BCL2L1 (rh Bcl-XL / BCL2L1; Catalog#10455-H08E; NP_612815.1; Met 1-Arg 212). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Specificity | Human Bcl-XL / BCL2L1 |
| No cross-reactivity in ELISA with E.coli cell lysate | |
| Western blot | This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human BCL2L1 in WB. Using a DAB detection system, the detection limit for Human BCL2L1 is approximately 1 ng/lane under non-reducing conditions and reducing conditions. |
| Direct ELISA | This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human BCL2L1. The detection limit for Human BCL2L1 is approximately 0.039 ng/well. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca (2+) to regulate both autophagy and apoptosis. Thus, Bcl-2 and Bcl-xL represent a molecular link between autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. Key regulators of apoptosis during anoxia are the Bcl-2 family of proteins. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2/Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be an inhibitor in cell death.
Sino Biological Inc. liefert Bcl-XL / BCL2L1 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.
Weitere Informationen über Bcl-XL / BCL2L1 Antikörper lesen Sie bitte: http://www.sinobiological.com/Bcl-XL-BCL2L1-Antibody-g-9162.html
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