Arylsulfatase A / ARSA Antikörper

24.08.2012

Arylsulfatase A / ARSA Antibody Product Information

Immunogen	Recombinant Mouse Arylsulfatase A / ARSA protein (Catalog#50018-M08H)
Antibody Type	Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
Clone ID	001
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Arylsulfatase A / ARSA (rM Arylsulfatase A / ARSA; Catalog#50018-M08H; NP_033843.2; Met 1-Ser 506).

Usage Guide von Arylsulfatase A / ARSA Antikörper

Specificity	Mouse Arylsulfatase A / ARSA
	No cross-reactivity in ELISA with Human ARSA
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse ARSA in WB. Using a DAB detection system, the detection limit for Mouse ARSA is approximately 0.5 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ARSA. The detection limit for Mouse ARSA is approximately 0.00975 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von Arylsulfatase A / ARSA Antikörper

Related Areas:

Enzyme>>Carbohydrate Metabolism Enzymes>>Arylsulfatase A/ARSA

Immunology>>Innate Immunity>>Lysosomal Enzyme>>Arylsulfatase A/ARSA

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
Arylsulfatase A	Human	Arylsulfatase A Protein, Recombinant	10449-H08H
Arylsulfatase A	Mouse	Arylsulfatase A Protein, Recombinant	50018-M08H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human Arylsulfatase A	WB, ELISA	Arylsulfatase A Antibody, Rabbit PAb	10449-RP01
Human Arylsulfatase A	WB, ELISA	Arylsulfatase A Antibody, Rabbit PAb (Antigen Affinity Purified)	10449-RP02
Human Arylsulfatase A	WB	Arylsulfatase A / ARSA Antibody	10449-R001
Human Arylsulfatase A	ELISA	Arylsulfatase A / ARSA Antibody	10449-R012
Mouse Arylsulfatase A	WB, ELISA	Arylsulfatase A Antibody, Rabbit PAb	50018-RP01
Mouse Arylsulfatase A	WB, ELISA	Arylsulfatase A Antibody, Rabbit PAb (Antigen Affinity Purified)	50018-RP02
Mouse Arylsulfatase A	WB, ELISA	Arylsulfatase A / ARSA Antibody	50018-R001

Hintergrund von Arylsulfatase A / ARSA Antikörper

Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme, and its major natural substrate is cerebroside 3-sulfate which is the constituent of the myelin sheet and is presented to the enzyme as complex with the sphingolipid activator protein B. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system.

References

Stein, C. et al., 1989, J. Biol. Chem. 264: 12.
Schmidt, B. et al., 1995, Cell. 82: 271-278.
Lukatela, G. et al., 1998, Biochemistry. 37: 3654-3664.
Von Bulow, R. et al., 2002, J. Biol. Chem. 277: 9455-9461.
Barth, M.L. et al., 1995, Hum. Mutat. 6: 170-176.

Sino Biological Inc. liefert Arylsulfatase A / ARSA Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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