APOLI / APOL1 Protein (His Tag)

09.10.2012

Produkt-Information von APOLI / APOL1 Protein

Synonym	APOL1
Protein Construction	A DNA sequence encoding the human APOL1 ( Met 1- Leu398) (Q2KHQ6) was expressed, with a C-terminal polyhistidine tag.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von APOLI / APOL1 Protein

Purity	> 91 % as determined by SDS-PAGE	SDS-PAGE: APOLI rotein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu 28
Molecular Mass	The secreted recombinant human APOL1 consists of 381 amino acids and predicts a molecular mass of 42.5 KDa. The apparent molecular mass of the protein is approximately 44 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 7.4, 10%gly, 3mM DTT. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von APOLI / APOL1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von APOLI / APOL1 Protein

APOL1, also known as apolipoprotein L1, is a member of the apolipoprotein L family. It is a minor apoprotein component of HDL (High-density lipoprotein) or 'good cholesterol' which is synthesized in the liver and also in many other tissues, including pancreas, kidney, and brain. APOL1 circulating in plasma is able to kill the trypanosome Trypanosoma brucei that causes sleeping sickness. It may play a role in lipid exchange and transport throughout the body. It may also participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). APOL1 is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.

References

Genovese G. et al., 2010, Science. 329 (5993): 841-5.
Tzur S. et al., 2010, Human Genetics. 128 (3): 345-50.
Hu CA. et al., 2012), FEBS Lett. 586 (7): 947-55.
Papeta N. et al., 2011, J Am Soc Nephrol. 22 (11): 1991-6.
Fine DM. et al., 2012, J Am Soc Nephrol. 23 (2): 343-50.

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