ADAM15 / MDC15 Protein (His Tag)

Produkt-Information von ADAM15 / MDC15 Protein

QC Testing von ADAM15 / MDC15 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: ADAM15 / MDC15 protein
Endotoxin	<1.0 EU per μg protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asp 207 (mature form)
Molecular Mass	The secreted recombinant human ADAM15 (mature form) comprises 501 amino acids after proteolytic of the signal peptide and pro peptide and has a predicted molecular mass of 54 kDa. As a result of glycosylation, rhADAM15 migrates as an approximately 65-70 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ADAM15 / MDC15 Protein

Verwandte Produkte & Themen von ADAM15 / MDC15 Protein

Enzyme>>Protease & Regulator>>Metalloprotease & Regulator>>ADAM / ADAMTS>>ADAM15

Cancer>>Angiogenesis>>ADAM / ADAMTS>>ADAM15

Beschreibung von ADAM15 / MDC15 Protein

The adamalysin ( ADAM ) metalloproteinase disintegrins, also known as metalloprotease/disintegrin/cysteine-rich proteins, are a branch of the metzincin metalloproteinase superfamily that are related to snake venom metalloproteinases and integrin ligands. ADAM15 was first named metargidin, and uniquely, it carries an RGD binding motif in a position similar to that in snake venom disintegrins. ADAM15 is widely expressed in various tissues and cells, including human umbilical vein endothelial cells and smooth muscle cells, and its expression can be drived by Vascular endothelial (VE)-cadherin. Overexpression of ADAM15 in NIH3T3 cells appears to enhance cell-cell interactions, as suggested by decreased cell migration, altered cell morphology at the wound edge, decreased monolayer permeability, and increased cell adhesion to monolayers of cells expressing ADAM15 by retroviral transduction. ADAM15 plays a physiological role as a natural binding partner of integrin αvβ3 thereby loosening tumor cell adhesion to the underlying matrix and regulating tumor cell migration and invasion, and functional interplay between ADAM15 and Src family PTKs may contribute to signaling in hematopoietic cells. Aslo, ADAM15 is believed to interact with integrins αvβ3, α5β1, and α9β1 through its disintegrin domain. ADAM15 has been shown to degrade collagens I and IV and to cleave the inflammatory cytokine CD23, thus influence ECM remodeling within rheumatoid synovial tissues and in atherosclerosis.The high expression levels of ADAM15 correlated with advanced metastatic diseases, and a clear association with clinical parameters was determined.

References

1. Barbara Herren, et al., 2001, Experimental Cell Research. 271 (1): 152-60.
2. Zaruhi Poghosyan, et al., 2002, Journal of Biologica Chemistry. 277 (7): 4999-5007.
3. Claire Ham. et al., 2002, Experimental Cell Research. 279 (2): 239-47.
4. Veronika Beck. et al., 2005, The International Journal of Biochemistry & Cell Biology. 37 (3): 590-603.
5. Jean-Fran?ois?Mosnier. et al., 2006, Laboratory Investigation. 86:1064-73.
6. Barbara Herren. et al., 1997, FASEB J. 11: 173-80.
7. Xi-Ping Zhang. et al., 1998, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 273 (13): 7345-50.
8. Rainer Kuefer. et al., 2006, Neoplasia. 8(4): 319-29.

Sino Biological Inc. liefert ADAM15 / MDC15 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ADAM15 / MDC15 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/ADAM15-MDC15-Protein-g-8823.html