VDR / NR111 Protein (His Tag)

28.03.2012

Produkt-Information von VDR / NR111 Protein

Synonym	NR1I1
Protein Construction	A DNA sequence encoding the human VDR (P11473) （Met 1-Ser 427）was fused with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von VDR / NR111 Protein

Purity	> 88 % as determined by SDS-PAGE	SDS-PAGE VDR / NR111 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met 1
Molecular Mass	The recombinant human VDR consists of 437 amino acids and migrates as an approximately 50 KDa band in SDS-PAGE in SDS-PAGE under reducing conditions as predicted.
Formulation	Lyophilized from 0.2μm filtered solution of 50mM Tris,100mM NaCl, pH 8.0, 10%glycerol Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

VDR / NR111 Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von VDR / NR111 Protein

VDR(vitamin D(1,25- dihydroxyvitamin D3)receptor, Histidine-tagged) , also known as NR1I1, belongs to the NR1I family, NR1 subfamily. It is comprised by three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. VDR can be detected in the intestine, thyroid and kidney and has a vital role in calcium homeostasis. VDR functions as nuclear hormone receptor and mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. It regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. VDR also plays a central role in calcium homeostasis and functions as a receptor for the secondary bile acid lithocholic acid. Defects in VDR are the cause of rickets vitamin D-dependent type 2A. A disorder of vitamin D metabolism can cause severe rickets, hypocalcemia and secondary hyperparathyroidism.

References

Moore DD. et al., 2006, Pharmacol Rev. 58 (4): 742-59.
Szpirer J. et al., 1991, Genomics. 11 (1): 168-73.
Germain P. et al., 2006, Pharmacol Rev. 58 (4): 685-704.
Adorini L. et al., 2006, Curr Top Med Chem. 6 (12): 1297-301.
Luderer HF. et al., 2010, J Steroid Biochem Mol Biol. 121 (1-2): 314-6.

Sino Biological Inc. liefert VDR / NR111 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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