TMEM27 Protein

20.12.2012

Sino Biological Inc. liefert TMEM27 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über TMEM27 Protein lesen Sie bitte: http://www.sinobiological.com/TMEM27-Protein-g-10167.html

Produkt-Information von TMEM27 Protein

Synonym	TMEM27
Protein Construction	A DNA sequence encoding the human TMEM27(Q9HBJ8) (Met1-Pro141) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von TMEM27 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: TMEM27 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu 15
Molecular Mass	The recombinant human TMEM27 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 368 amino acids and has a predicted molecular mass of 41.4 kDa. The apparent molecular mass of the protein is approximately 53-57 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von TMEM27 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von TMEM27 Protein

TMEM27 is a membrane protein. It forms dimers and its extracellular domain is glycosylated, cleaved and shed from the plasma membrane of beta cells. Thus it has been proposed as a beta cell mass biomarker. Overexpression of TMEM27 leads to increased thymidine incorporation, whereas silencing of Tmem27 using RNAi results in a reduction of cell replication. Furthermore, transgenic mice with increased expression of Tmem27 in pancreatic beta cells exhibit increased beta cell mass. TMEM27 is important for trafficking amino acid transporters to the apical brush border of proximal tubules.

References

Pasquali L. et al., 2009, J Genet. 88 (1): 105-8.
Tosetto E. et al., 2009, Pediatr Nephrol. 24 (10): 1967-73.
Singer D. et al., 2011, Channels (Austin). 5 (5): 410-23.