Tie1 ELISA Pair Set, Human ELISA Pair Set

Fängerantikörper 0.5 mg/mL of mouse anti-Tie1 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating..

Nachweisantikörper 0.5 mg/mL mouse anti-Tie1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 160 ng of recombinant Tie1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.

The minimum detectable dose of Human Tie1 was determined to be approximately 78 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Human Tie1 ELISA Pair Set is for the quantitative determination of Human Tie1.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Tie1 coated on a 96-well plate. Standards and samples are added to the wells, and any Tie1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Tie1 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Tie1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

Tyrosine-protein kinase receptor Tie-1, also known as TIE1, is a single-pass type I membrane protein which belongs to the protein kinase superfamily, Tyr protein kinase family and Tie subfamily. TIE1 contains three EGF-like domains, three fibronectin type-III domains, two Ig-like C2-type ( immunoglobulin-like ) domains and one protein kinase domain. TIE1 is an endothelial-cell-specific tyrosine kinase receptor. It is a cell surface protein expressed exclusively in endothelial cells. It is required for the survival and growth of microvascular endothelial cells during the capillary sprouting phase of vascular development. TIE1 is an orphan receptor tyrosine kinase that is expressed almost exclusively in endothelial cells and that is required for normal embryonic vascular development. TIE1 upregulates the cell adhesion molecules (CAMs) VCAM-1, E-selectin, and ICAM-1 through a p38-dependent mechanism. Attachment of monocyte derived immune cells to endothelial cells is also enhanced by TIE1 expression. TIE1 has a proinflammatory effect and may play a role in the endothelial inflammatory diseases such as atherosclerosis.