ST6GAL1 / CD75 Antikörper

30.08.2012

Produkt-Informaiton von ST6GAL1 / CD75 Antikörper

Immunogen	Recombinant Human ST6GAL1 / CD75 protein (Catalog#11590-H07H)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	09
Ig Type	Mouse IgG1
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ST6GAL1 / CD75 (rh ST6GAL1 / CD75; Catalog#11590-H07H; P15907; Lys 27-Cys406). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von ST6GAL1 / CD75 Antikörper

Specificity	Human ST6GAL1 / CD75
	No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human ST6GAL1 in WB. Using a DAB detection system, the detection limit for Human ST6GAL1 is approximately 4 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von ST6GAL1 / CD75 Antikörper

Related Areas:

Enzyme>>Carbohydrate Metabolism Enzymes>>ST6GAL1/CD75

Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>ST6GAL1/CD75

Immunology>>Adaptive Immunity>>T Cell>>T Cell Migration/Adhesion>>ST6GAL1/CD75

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
ST6GAL1/CD75	Human	ST6GAL1/CD75 Protein, Recombinant	11590-H07H
ST6GAL1/CD75	Mouse	ST6GAL1 / CD75 Protein, Recombinant	50740-M07H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human ST6GAL1 / CD75	ELISA	ST6GAL1 / CD75 Antibody	11590-MM05
Human ST6GAL1 / CD75	WB	ST6GAL1 / CD75 Antibody	11590-MM09
Human ST6GAL1 / CD75	WB, ELISA	ST6GAL1 / CD75 Antibody (Antigen Affinity Purified)	11590-RP02

Hintergrund von ST6GAL1 / CD75 Antikörper

Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein which belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in theGolgibut which can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.

References

Collins,B.E. et al., 2006, Nat Immunol. 7 (2): 199-206.
Videira,P.A. et al., 2008, Glycoconj J. 25 (3): 259-68.
Petit,D. et al., 2010, J Biol Chem. 285 (49): 38399-414.
Kroes,R.A. et al., 2010, Proc Natl Acad Sci USA. 107 (28): 12646-51.

Sino Biological Inc. liefert ST6GAL1 / CD75 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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