SerpinI2 Antikörper (Antigen Affinität reinigt)

05.03.2012

Produkt-Information von SerpinI2 Antikörper

Immunogen	Recombinant Human SerpinI2 protein (Catalog#11008-H08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Human SerpinI2 (rh SerpinI2;Catalog#11008-H08H; NP_006208.1; Met 1-Leu 405). SerpinI2 specific IgG was purified by Human SerpinI2 affinity chromatography.

SerpinI2 Antikörper Usage Guide

Specificity	Human SerpinI2
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SERPINI2 in WB. Using a DAB detection system, the detection limit for Human SERPINI2 is approximately 0.5 ng/lane under non-reducing conditions reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SERPINI2. The detection limit for Human SERPINI2 is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von SerpinI2 Antikörper

Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases (serine protease inhibitors). Over 1000 serpins have been identified. Serpin-I2, also known as myoepithelium-derived serine protease inhibitor, Pancreas-specific protein TSA2004, Peptidase inhibitor 14, PI14, SERPINI2 and MEPI, is a secreted protein which belongs to theserpin family. It is expressed in pancreas and adipose tissues. SERPINI2 deficiency directly results in the acinar cell apoptosis and malabsorption.

References

Riewald M. et al., 1995, J. Biol. Chem. 270: 26754-7.
Forsyth, S. et al., 2003, Genomics 81: 336-45.
Horvath, AJ. et al., 2004, J. Mol. Evol. 59: 488-97.
Loftus,S.K.et al., 2005, PLoS Genet. 1 (3): e38.
Steenbakkers PJ. et al., 2008, Mycol. Res.112 (Pt 8): 999-1006.
Przygodzka, P. et al., 2010, BMC Cell Biol. 11: 30.

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