RNASET2 Protein (His Tag)

19.04.2012

Produkt-Information von RNASET2 Protein

Synonym	RP11-514O12.3, RNASE6PL, bA514O12.3
Protein Construction	A DNA sequence encoding the human RNASET2 isoform 1 (O00584-1) (Met 1- His 256) was fused with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von RNASET2 Protein

Purity	> 98 % as determined by SDS-PAGE	SDS-PAGE: RNASET2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asp 25
Molecular Mass	The secreted recombinant human RNASET2 comprises 242 amino acids and has a predicted molecular mass of 28.5 kDa. The apparent molecular mass of rh RNASET2 is approximately 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH7.4，10%gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von RNASET2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von RNASET2 Protein

RNASET2 (ribonuclease T2) is an enzyme which belongs to the RNase T2 family. Highly expression of RNASET2 can be found in the temporal lobe and fetal brain. It is encoded by the RNASET2 gene. This ribonuclease gene is a novel member of the Rh/T2/S-glycoprotein class of extracellular ribonucleases. It is a single copy gene that maps to 6q27, a region associated with human malignancies and chromosomal rearrangement. RNASET2 deficiency may interfere with brain development and myelination through angiogenesis or RNA metabolism. It behaves as a class II tumor suppressor gene for ovarian cancer, since experimental overexpression of this gene in human ovarian cancer cell lines is associated with a significant decrease of their tumorigenic and metastasizing potential in vivo. Strikingly, the ribonuclease catalytic activity is apparently dispensable for RNASET2 to play such antioncogenic role. Indeed, a double CAS mutant cDNA construct encoding an almost inactive RNASET2 protein is still able to suppress tumorigenicity and metastasis when overexpressed in the same ovarian cancer cell lines.

References

Acquati F. et al., 2011, Oncotarget. 2 (6): 477-84.
Campomenosi P. et al., 2011, Biotechnol Appl Biochem. 58 (1): 39-49.
Monti L. et al., 2008, Oncol Res. 17 (2): 69-74.

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