RHEB Protein (His Tag)

17.04.2013

Sino Biological Inc. liefert RHEB Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über RHEB Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/RHEB-RHEB2-Protein-Antibody-a-5848.html

Produkt-Information von RHEB Protein

Synonym	RHEB, RHEB2
Protein Construction	A DNA sequence encoding the human RHEB (Q15382)(Met1-Cys181) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von RHEB Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: RHEB protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human RHEB consists of 199 amino acids and has a calculated molecular mass of 22.4 kDa. The recombinant protein migrates as an approximately 21 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von RHEB Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von RHEB Protein

RHEB is a recently discovered member of the Ras superfamily that may be involved in neural plasticity. This function is novel and not typically associated with the Ras proteins. RHEB gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is vital in regulation of growth and cell cycle progression due to its role in the insulin / TOR / S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of RHEB is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.

References

Karbowniczek. et al., 2004, J Biol Chem. 279 (29): 29930-7.
Long. et al., 2005, Curr Biol. 15 (8): 702-13.
Mizuki N. et al., 1996, Genomics. 34 (1): 114-8.

RHEB (RHEB2) related areas, pathways, and other information