Rat NINJURIN / Ninjurin-1 / NINJ1 Protein

08.05.2012

Produkt-Information von NINJURIN / Ninjurin-1 / NINJ1 Protein

Synonym	NINJ1
Protein Construction	A DNA sequence encoding the rat NINJ1 (P70617) extracellular domain (Met 1-Pro 79) was fused with the Fc region of human IgG1 at the N-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von NINJURIN / Ninjurin-1 / NINJ1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: NINJURIN / Ninjurin-1 / NINJ1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu
Molecular Mass	The recombinant rat NINJ1/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 340 amino acids and has a calculated molecular mass of 37 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rat NINJ1/Fc monomer is approximately 50 kDa due to glycosylation.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von NINJURIN / Ninjurin-1 / NINJ1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NINJURIN / Ninjurin-1 / NINJ1 Protein

NEIL1 belongs to the DNA glycosylases family. DNA glycosylases are enzymes that participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. They initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 also has marginal activity towards 8-oxoguanine and AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches.

References

Zhang QM. et al., 2005, DNA Repair. 4 (1): 71-9.
Mokkapati SK. et al., 2004, Biochemistry. 43 (36): 11596-604.
Shinmura K. et al., 2005, Carcinogenesis. 25 (12): 2311-7.
Doublié S. et al., 2004, Proc Natl Acad. 101 (28): 10284-9.

Sino Biological Inc. liefert Rat NINJURIN / Ninjurin-1 / NINJ1 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat NINJURIN / Ninjurin-1 / NINJ1 Protein lesen Sie bitte: http://www.sinobiological.com/NINJURIN-Ninjurin-1-NINJ1-Protein-g-9034.html