Rat Cadherin-5 / CDH5 / VE-Cad Protein

11.03.2013

Sino Biological Inc. liefert Rat Cadherin-5 / CDH5 / VE-Cad Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat Cadherin-5 / CDH5 / VE-Cad Protein lesen Sie bitte: http://www.sinobiological.com/Cadherin-5-CDH5-VE-Cad-Protein-g-10565.html

Produkt-Information von Cadherin-5 / CDH5 / VE-Cad Protein

Synonym	CDH5
Protein Construction	A DNA sequence encoding the rat CDH5 (Met1-Gln585) was expressed with a polyhistidine tag at the C-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von Cadherin-5 / CDH5 / VE-Cad Protein

Purity	> 75 % as determined by SDS-PAGE	SDS-PAGE: Cadherin-5 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly 25
Molecular Mass	The recombinant rat CDH5 comprises 802 amino acids and predicts a molecular mass of 90.7 kDa. The apparent molecular mass of the recombinant protein is approximately 116 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von Cadherin-5 / CDH5 / VE-Cad Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Cadherin-5 / CDH5 / VE-Cad Protein

Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. They play important roles in cell adhesion, ensuring that cells within tissues are bound together. They are dependent on calcium(Ca2+) ions to function. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, is single-pass type I membrane protein. It is expressed in endothelial tissues and brain and is involved in loss of heterozygosity events in breast and prostate cancer. CDH5 is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, CDH5 may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.

References

Angst B. et al., 2001, J Cell Sci. 114 (4): 629-41.
Duguay D. et al., 2003, Dev Biol. 253: 309-23.
Bayas MV. et al., 2005, Biophysical Journal. 90 (4): 1385.
Crosby CV, et al., 2005, Blood. 105 (7): 2771-6.
Hulpiau P. et al., 2009, Int J Biochem Cell Biol. 41 (2): 349-69.