PTMA Protein (GST Tag)

27.03.2012

Produkt-Information von PTMA Protein

Synonym	TMSA
Protein Construction	A DNA sequence encoding the human PTMA (P06454-1) (Ser 2-Asp 111) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	E.coli

PTMA Protein QC Testing

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: PTMA protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human PTMA/GST chimera consists of 341 amino acids and has a predicted molecular mass of 39 kDa. It migrates as an approxiamtely 45 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH 7.5 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

PTMA Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PTMA Protein

PTMA prothymosin, alpha,,N-GST chimera) is a small, 12.4 kDa protein. It is a 109-111 amino acid long polypeptide as the precursor of thymosin alpha-1. Normally, PTMA protein can be seen in nuclei of normal cells in various mammalian tissues. PTMA protein is also detected in the cytoplasm. In general, it is associated with cellular proliferation and carcinogenesis, cellular and viral transcription (Cotter et al., 2000), protection against apoptosis and chromatin remodelling. It may have a dual role both intracellulary and extracellulary. The intracellular roles of PTMA are 1) cell proliferation / differentiation, 2) chromatin remodelling, 3) antiapoptotic activity through inhibition of apoptosome formation, and so on. The extracellular roles of PTMA are 1) immunoenhancing activity by stimulating immune responses, and so forth. PTMA is required for cell division, growth, and survival (Piñeiro et al., 2000). It is also up-regulated in proliferating, transcriptionally active cells. PTMA gene level is elevated in normal proliferating tissue, but repressed in quiescent cells.

References

Manrow RE. et al, 1992, Genomics. 13 (2): 319-31.
Wara DW. et al., 1975,N Engl J Med. 292 (2): 70-4.
Garaci E. et al., 2007, Ann N Y Acad Sci. 1112: 225-34.
Goldstein AL. et al., 2009, Expert Opin Biol Ther. 9 (5): 593-608.

Sino Biological Inc. liefert PTMA Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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