Phosphomannomutase 2 / PMM2 / CDG1 Protein (His Tag)

29.05.2013

Sino Biological Inc. liefert Phosphomannomutase 2 / PMM2 / CDG1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Phosphomannomutase 2 / PMM2 / CDG1 Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/Phosphomannomutase-2-PMM2-CDG1-Protein-Antibody-a-6211.html

Produkt-Information von Phosphomannomutase 2 / PMM2 / CDG1 Protein

Synonym	PMM2
Protein Construction	A DNA sequence encoding the human PMM2 (O15305) (Met1-Ser246) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von Phosphomannomutase 2 / PMM2 / CDG1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: PMM2 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human PMM2 consists of 261 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 28 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris,100mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von Phosphomannomutase 2 / PMM2 / CDG1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Phosphomannomutase 2 / PMM2 / CDG1 Protein

Phosphomannomutase 2, also known as PMM2 and CDG1, belongs to the eukaryotic PMM family. Phosphomannomutase 2 catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate. Mannose 1-phosphate is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. GDP-mannose can transfer its small sugar molecule called mannose to the growing oligosaccharide chain. Once the correct number of small sugar molecules are linked together to form the oligosaccharide, it can be attached to a protein. Phosphomannomutase 2 is also required for a number of critical mannosyl transfer reactions. Mutations in PMM2 gene have been shown to cause defects in the protein glycosylation pathway manifest as carbohydrate-deficient glycoprotein syndrome type I.

References

Jaeken J. et al., 2002, Annual review of genomics and human genetics. 2: 129-51.
Matthijs G. et al., 2000, Mol Genet Metab. 68 (2): 220-6.
Matthijs G. et al., 1997, Nat Genet. 16 (1): 88-92.