NBL1 / DAND1 / DAN ELISA Pair Set, Human ELISA Pair Set

Fängerantikörper 0.5 mg/mL of mouse anti-DAN monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL mouse anti-DAN monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 110 ng of recombinant DAN. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.

The minimum detectable dose of Human NBL1 / DAND1 / DAN was determined to be approximately 46.9 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Human NBL1 / DAND1 / DAN ELISA Pair Set is for the quantitative determination of Human NBL1 / DAND1 / DAN.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for NBL1 / DAND1 / DAN coated on a 96-well plate. Standards and samples are added to the wells, and any NBL1 / DAND1 / DAN present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-NBL1 / DAND1 / DAN monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of NBL1 / DAND1 / DAN present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

Human differential screening-selected gene aberrative in neuroblastoma (DAN) originally isolated from a normal lung cDNA library, is a the prototypical member of the DAN family of secreted glycoproteins. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN acts as the BMPs (bone morphogenetic protein) antagonist by binding to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. In addition, it has been reported that DAN also acts as an inhibitor of cell cycle progression and monocyte chemotaxis, and is closely involved in human neuroblastoma differentiation. DAN thus is regarded as a novel candidate of tumour suppressor.