Mouse PHKG2 cDNA gene Clone / ORF gene clone

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

 1 ATGACGTTGG ACGTGGGGCC GGAGGATGAA CTCCCTGACT GGGCTGCGGC CAAAGAGTTT 61 TACCAGAAGT ACGACCCTAA GGACATCATT GGCAGAGGAG TGAGCTCTGT GGTCCGCCGC 121 TGTGTCCATC GAGCTACCGG TGATGAGTTC GCAGTGAAGA TCATGGAGGT GTCAGCTGAG 181 CGGCTGAGTC TTGAGCAGCT GGAGGAGGTG CGGGATGCCA CACGGCGAGA GATGCACATT 241 CTTCGCCAGG TCGCTGGCCA CCCCCATATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT 301 AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAAGGAG AGCTGTTTGA CTATCTCACC 361 GAGAAAGTGG CCCTCTCAGA AAAGGAAACC AGGTCCATCA TGAGGTCTCT ACTGGAAGCA 421 GTGAGCTTTC TCCATGCCAA CAACATTGTA CATCGAGACC TGAAGCCTGA GAATATTCTC 481 CTAGATGACA ATATGCAGAT ACGCCTTTCA GATTTTGGGT TCTCCTGCCA TTTGGAAGCT 541 GGCGAGAAGC TTCGAGAACT GTGCGGGACT CCAGGGTATC TAGCACCAGA GATCCTTAAA 601 TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAAG TTGATCTCTG GGCTTGTGGG 661 GTGATCCTGT TCACACTTCT GGCTGGCTCA CCACCATTCT GGCACCGGCG CCAAATCCTG 721 ATGCTACGCA TGATCATGGA AGGCCAATAT CAGTTTACTT CACCTGAGTG GGATGATCGT 781 TCAAACACCG TCAAAGACCT GATCTCCAAG CTGCTGCAGG TGGATCCTGA AGCGCGCCTG 841 ACAGCTGAGC AAGCCCTGCA GCACCCCTTC TTTGAGCGCT GTGAAGGCAG CCAACCTTGG 901 AACCTCACGC CTCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAATACTGGC TGCCGGACGA 961 GTGGCCTTAA GCAGTCACCG TTTACGGCCA CTAACTAAGA ATGCACTATT GAGAGACCCC 1021 TATGCACTGC GGCCAGTACG GCGCCTCATC GACAACTGTG CCTTCCGGCT CTATGGGCAC 1081 TGGGTAAAGA AGGGTGAGCA GCAGAACCGG GCAGCCCTCT TCCAGCACCA GCCTCCTAGG 1141 CTGTTCCCCA TTGCTGCCAC TGAACTCGAA GGAGACTCCG GTGCCATCAC AGAGGATGAG 1201 GCTACACTGG TACGGAGCTA G 

Quality Control:cDNA sequencing results is available upon request.