LMAN2 / VIP36 Antikörper

05.07.2012

Produkt-Information von LMAN2 / VIP36 Antikörper

Immunogen	Recombinant Human LMAN2 / VIP36 protein (Catalog#12455-H08E)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	02
Ig Type	Mouse IgG2a
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LMAN2 / VIP36 (rh LMAN2 / VIP36; Catalog#12455-H08E; Q12907; Asp 45-Arg 322). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von LMAN2 / VIP36 Antikörper

Specificity	Human LMAN2 / VIP36
	No cross-reactivity in ELISA with E.coli cell lysate
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human LMAN2 in WB. Using a DAB detection system, the detection limit for Human LMAN2 is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
Direct ELISA	This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human LMAN2. The detection limit for Human LMAN2 is approximately 0.078 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

LMAN2 / VIP36 Antikörper

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Antibodies:

Hintergrund von LMAN2 / VIP36 Antikörper

LMAN2 (Lectin, mannose-binding 2), also known as VIP36 (36 kDa vesicular-integral membrane protein), has been originally isolated from MDCK cells as a component of glycolipid-enriched detergent-insoluble complexes containing apical marker proteins. VIP36 binds glycoproteins that retain the intact D1 mannosyl branch in the cis-Golgi network and recycles to the endoplasmic reticulum where, due to higher pH, it releases its cargos, thereby contributing to the quality control of glycoproteins. VIP36 is an intracellular lectin that cycles between the endoplasmic reticulum (ER) and the Golgi apparatus, and is thought to act as a cargo receptor in the transport and sorting of glycoproteins. The interaction between recombinant soluble VIP36 and BiP is dependent on divalent cations but not on ATP.

References

Hara-Kuge S. et al., 1999, Glycobiology. 9 (8): 833-9.
Kamiya Y. et al., 2005, J Biol Chem. 280 (44): 37178-82.
Nawa D. et al., 2007, Glycobiology. 17 (9): 913-21.

Sino Biological Inc. liefert LMAN2 / VIP36 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über LMAN2 / VIP36 Antikörper lesen Sie bitte: http://www.sinobiological.com/LMAN2-VIP36-Antibody-g-9174.html