IZUMO4 / C19orf36 Protein

10.09.2012

Produkt-Information von IZUMO4 / C19orf36 Protein

Synonym	C19orf36, IZUMO4
Protein Construction	A DNA sequence encoding the human C19orf36 (Q1ZYL8)(Met1-His214) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von IZUMO4 / C19orf36 Protein

Purity	> 96 % as determined by SDS-PAGE	SDS-PAGE: IZUMO4 / C19orf36 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Cys 18
Molecular Mass	The recombinant human C19orf36/Fc comprises 438 amino acids and has a predicted molecular mass of 49.8 kDa. The apparent molecular mass of the protein is approximately 55-59 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von IZUMO4 / C19orf36 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von IZUMO4 / C19orf36 Protein

IZUMO4 has an N-terminal domain with significant homology to the N-terminal domain of Izumo. Izumo is a sperm membrane protein which plays a key role in the fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain(Izumo domain) for which neither the functions nor homologous sequences are known. Besides IZUMO4, there are two other novel proteins which also have a Izumo domain, they are IZUMO2 and IZUMO3. Izumo 1-3 are transmembrane proteins expressed specifically in the testis, and Izumo 4 is a soluble protein expressed in the testis and in other tissues. Izumo 1, 3, and 4 formed protein complexes on sperm, Izumo 1 forming several larger complexes and Izumo 3 and 4 forming a single larger complex. Izumo domain possesses the ability to form dimers, whereas the transmembrane domain or the cytoplasmic domain or both of Izumo 1 are required for the formation of multimers of higher order. Co-immunoprecipitation studies showed the presence of other sperm proteins associated with Izumo 1, suggesting Izumo 1 forms a multiprotein membrane complex.

References

Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
Lamesch P. et al., 2007, Genomics. 89 (3): 307-15.
Ellerman DA. et al., 2009, Mol Reprod Dev. 76 (12): 1188-99.

Sino Biological Inc. liefert IZUMO4 / C19orf36 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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