ICAM-1 / CD54 Antikörper ( FITC )

20.01.2012

Produkt-Information von ICAM-1 / CD54 Antikörper

Immunogen :	Recombinant Human ICAM-1 protein (Catalog#10346-H08H)
Reagents :	FITC-conjugated mouse monoclonal antibody
Clone ID :	8B9H12
Ig Type :
Concentration :	5 μl/Test, 0.2 mg/ml
Formulation :	Aqueous solution containing 0.5% BSA and 0.1% sodium azide
Preparation :	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM-1 / CD54 (rh ICAM-1 / CD54; Catalog#10346-H08H; NP_000192.2; Met 1-Glu 480) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

ICAM-1 / CD54 AntikörperUsageGuide

Specificity :	Human ICAM-1 / CD54
	No cross-reactivity in ELISA with Human ICAM2 Human ICAM3 Human VCAM1 Human CD62E Human CEACAM1 Human cell lysate (293 cell line)
Flow Cytometry :	1. Profile of anti-ICAM1(CD54) reactivity on Daudi cells analyzed by flow cytometry. Fig 1, Flow cytometric analysis of anti-ICAM1(CD54) reactivity on Daudi cells 2. HUVEC cells (Human umbilical vein endothelial cells) were treated with TNF-α (50 ng/ml,18 hours). The cells were harvested and stained with FITC Mouse anti-Human ICAM1 (10346-MM01). Fig 2, Flow cytometric analysis of Human ICAM1 (CD54) in HUVEC cells Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Storage :	This antibody is stable for 24 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Hintergrund von ICAM-1 / CD54 Antikörper

ICAM1 (Inter-Cellular Adhesion Molecule 1), also known as CD54, is a type of intercellular adhesion molecule constitutely present in low concentrations in the membranes of leukocytes and endothelial cells. As an endothelial and leukocyte-associated transmembrane protein, ICAM1 is well known for its importance in stabilizing cell-cell interactions and facilitating leukocyte endothelial transmigration.ICAM1 is a ligand for LFA-1 (integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease.

References

Rothlein, R. et al.,1986, Journal of Immunology. 137 (4): 1270-1274.
Yang, L. et al., 2005, Blood. 106 (2): 584-592.
Frijns, C.J. et al., 2002, Stroke. 33 (8): 2115-2122.
Bella, J.et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95 (8): 4140-4145.

Sino BIological Inc. liefert ICAM-1 / CD54 Antikörper ( FITC ) mit höhere Qualität und günstigen Kosten im Vergleich mit anderen globalen Lieferanten.

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ICAM-1 / CD54 Antikörper ( FITC )

Produkt-Information von ICAM-1 / CD54 Antikörper

ICAM-1 / CD54 AntikörperUsageGuide

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Hintergrund von ICAM-1 / CD54 Antikörper

References