HGFR / c-MET Antikörper

03.07.2012

Produkt-Information von HGFR / c-MET Antikörper

Immunogen	Recombinant Canine HGFR / c-MET protein (Catalog#70008-D08H)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	05
Ig Type	Mouse IgG1
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Canine HGFR / c-MET (rCanine HGFR / c-MET; Catalog#70008-D08H; NP_001002963.1; Met 1-Leu 935). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von HGFR / c-MET Antikörper

Specificity	Canine HGFR / c-MET
	No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Canine c-MET in WB. Using a DAB detection system, the detection limit for Canine c-MET is approximately 20 ng/lane under non-reducing conditions and not recommended under reducing conditions.
Direct ELISA	This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Canine c-MET. The detection limit for Canine c-MET is approximately 0.039 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von HGFR / c-MET Antikörper

Related Areas:

Enzyme>>Protein Kinase>>Receptor Tyrosine Kinase>>HGFR/c-MET

Signal Transduction>>Protein Kinase>>Receptor Tyrosine Kinase>>HGFR/c-MET

Cancer>>Cancer Biomarkers>>HGFR/c-MET

Cancer>>Growth Factor & Receptor>>Receptor Tyrosine Kinase>>HGFR/c-MET

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
HGFR/c-MET	Human	HGFR/c-MET/Fc Protein, Recombinant	10692-H03H
HGFR/c-MET	Human	HGFR/c-MET Protein, Recombinant	10692-H08H
HGFR/c-MET	Human	c-MET / HGFR (aa 956-1390) Protein, Recombinant, with GST Tag	10692-H20B1
HGFR/c-MET	Mouse	HGFR/c-MET/Fc Protein, Recombinant	50622-M02H
HGFR/c-MET	Mouse	HGFR/c-MET Protein, Recombinant	50622-M08H
HGFR/c-MET	Canine	HGFR/c-MET Protein, Recombinant	70008-D08H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human HGFR/c-MET	WB, ELISA	HGFR/c-MET Antibody, Mouse MAb	10692-MM02
Canine HGFR/c-MET	WB, ELISA	HGFR/c-MET Antibody, Rabbit PAb	70008-RP02
Canine HGFR/c-MET	WB, ELISA	HGFR / c-MET Antibody	70008-MM05

Hintergrund von HGFR / c-MET Antikörper

The proto-oncogene MET encoded product MET (mesenchymal-epithelial transition factor), also known as c-Met or hepatocyte growth factor receptor (HGFR), is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. As the prototypic member of a small subfamily of growth factor receptors, c-Met/HGFR is synthesized as a single chain precursor, and is processed into a mature disulfide-linked heterodimer composed of a extracellular α subunit and a transmembrane β subunit via post-translational cleavage. c-Met/HGFR is identified as a glycosylated receptor tyrosine kinase (RTK), and HGF is the only known ligand. Following ligand binding and autophosphorylation, c-Met/HGFR transmits intercellular signals using a unique multisubstrate docking site which mediates the binding of multiple SH2-containing adapter proteins such as Grb2, SHC, Crk/CRKL, as well as Gab1. Normal c-Met/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active c-Met/HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. Two transcript variants encoding different isoforms have been identified.

References

Bottaro, D.P. et al., 1991, Science. 251: 802-804.
Birchmeier, C. et al., 2003, Nat. Rev. Mol. Cell Biol. 4: 915-925.
Weidner, K.M. et al., 1996, Nature. 384: 173-176.
Pennacchietti, S. et al., 2003, Cancer. Cell. 3: 347-361.
Boccaccio, C. et al., 2006, Nat. Rev. Cancer. 6: 637-645.
Chmielowiec, J. et al., 2007, J.Cell. Biol. 177: 151-162.
Yu, Y.L. et al., 2002, Cancer. Res. 62: 2951-2956.
Furge, K.A. et al., 2000, Oncogene. 19: 5582-5589.

Sino Biological Inc. liefert HGFR / c-MET Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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