HGF / Hepatocyte Growth Factor Antikörper

01.08.2012

HGF / Hepatocyte Growth Factor Antikörper

Immunogen	Recombinant Human HGF / Hepatocyte Growth Factor protein (Catalog#10463-H08H)
Antibody Type	Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
Clone ID	007
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was obtained from a rabbit immunized with purified, recombinant Human HGF / Hepatocyte Growth Factor (rh HGF / Hepatocyte Growth Factor; Catalog#10463-H08H; NP_000592.3; Met 1-Ser 728).

Usage Guide von HGF / Hepatocyte Growth Factor Antikörper

Specificity	Human HGF / Hepatocyte Growth Factor
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human HGF in WB under reducing conditions and not recommended under non-reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HGF. The detection limit for Human HGF is approximately 0.00975 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Hintergrund von HGF / Hepatocyte Growth Factor Antikörper

Hepatocyte growth factor (HGF), also known as Scatter factor, is a paracrine cellular growth, motility and morphogenic factor. Hepatocyte Growth Factor/HGF is a member of the peptidase S1 family, and is derived from a single chain precursor of 728 amino acid residues containing 4 kringle domains, 1PAN domain and 1peptidase S1 domain. The synthesized single chain of HGF is further processed into a bioactive heterodimer composed of the 69 kDa α-subunit and the 34 kDa β-subunit by a serine protease. Hepatocyte Growth Factor/HGF has no detectable protease activity. Alternative splicing of its gene produces multiple transcript variants encoding different isoforms. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, as well as haemopoietic progenitor cells. Hepatocyte Growth Factor/HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. Hepatocyte Growth Factor/HGF may also play an important role as a paracrine or endocrine mediator through an epithelial-mesenchymal interaction in wound-healing, tissue or organ regeneration, morphogenesis and carcinogenesis.

References

Naldini, L. et al.,1991, EMBO J. (ENGLAND)10 (10): 2867-78.
Nakamura, T. et al., 1992,Prog. Growth Factor Res.3 (1): 67-85.
Comoglio, PM. et al., 1993, EXS. (SWITZERLAND)65: 131-65.
Skibinski, G. et al., 2004, Arch. Immunol. Ther. Exp. 51 (5): 277-82.
Kemp, LE. et al., 2006, Biochem. Soc. Trans.34 (Pt 3): 414-7.

Sino Biological Inc. liefert HGF / Hepatocyte Growth Factor Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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HGF / Hepatocyte Growth Factor Antikörper